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Fig. 2 | Canadian Journal of Kidney Health and Disease

Fig. 2

From: Early outgrowth pro-angiogenic cell number and function do not correlate with left ventricular structure and function in conventional hemodialysis patients: a cross-sectional study

Fig. 2

Assays of EPC function. Cultured EPCs were grown from peripheral blood mononuclear cells as described in the Methods section, and then stained with the isolectin B4 Ulex europaeus agglutinin I to measure their in vitro endothelial differentiation potential. Representative images are shown in (a). Original magnification 20X. In (b), the in vitro EPC differentiation potential of each individual patient is represented by a dot. In (c), 250,000 EPCs were seeded in inserts that were placed in wells of a Boyden companion plate containing VEGF 100 ng/mL in the lower chamber. The number of cells migrating through the 8 μm pore size insert was counted after 4 h. Each dot represents the value for an individual patient. Apoptosis of cultured EPCs was also quantified using TUNEL staining. In (d), each dot represents the percentage of apoptotic EPCs for an individual patient. In (e), representative TUNEL stained images are shown. Original magnification 20X

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